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Macroporous Scaffolds Based on Chitosan and Bioactive Molecules

Department of Biomaterials, Instituto de Ciencia y Tecnología de Polímeros Consejo Superior de Investigaciones Científicas (CSIC) Juan de la Cierva 3, 28006 Madrid, Spain

M.R. Aguilar

Department of Biomaterials, Instituto de Ciencia y Tecnología de Polímeros Consejo Superior de Investigaciones Científicas (CSIC) Juan de la Cierva 3, 28006 Madrid, Spain, mraguilar{at}ictp.csic.es

J. San Roman

Department of Biomaterials, Instituto de Ciencia y Tecnología de Polímeros Consejo Superior de Investigaciones Científicas (CSIC) Juan de la Cierva 3, 28006 Madrid, Spain

M. Fernandez

Department of Pharmacology, Facultad de Farmacia (UCM) Ciudad Universitaria s/n, 28040 Madrid, Spain

M.A. Prieto

Department of Molecular Microbiology, Centro de Investigaciones Biológicas Consejo Superior de Investigaciones Científicas (CSIC) C. Ramiro de Maeztu, 9, 28040 Madrid

B. Bondar

Institute of Pathology, University of Mainz, Langenbeckstr 1. Bldg. 402, D-55101 Mainz, Germany

Chitosan-based macroporous scaffolds for tissue engineering applications are developed by cryogelation in aqueous media. The cryogels obtained are modified using a new RGD-containing peptide developed in this laboratory. A RGD-containing peptide is chemically attached to the surface of the cryogels to improve cell adhesion to the 3D-structure chitosan-based scaffolds. The synthesis, physico-chemical, and biological evaluations of the system are described, and the optimization of the formulations is carried out by varying the reaction parameters. Fibroblasts and endothelial cells are used in cell cultures to determine cell behavior and the cytocompatibility of the macroporous cryogels. Cell spreading and actin cytoskeleton organization process are assessed by confocal microscopy. Cells colonize the porous structure of the chitosan-based cryogel and are observed to be growing inside the pores.

Key Words: chitosan • cytocompatibility • RGD • cryogels • scaffold • chitosan.

Journal of Bioactive and Compatible Polymers, Vol. 22, No. 6, 621-636 (2007)
DOI: 10.1177/0883911507084293


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