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Rapid Prototyping Three-Dimensional Cell/Gelatin/Fibrinogen Constructs for Medical Regeneration

Key Laboratory for Advanced Manufacturing by Materials Processing Technology & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University, Beijing 100084, P.R. China, wangxiaohong @tsinghua.edu.cn

Xiaohong Wang

Key Laboratory for Advanced Manufacturing by Materials Processing Technology & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University, Beijing 100084, P.R. China

Yongnian Yan

Key Laboratory for Advanced Manufacturing by Materials Processing Technology & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University, Beijing 100084, P.R. China

Wei Zheng

Key Laboratory for Advanced Manufacturing by Materials Processing Technology & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University, Beijing 100084, P.R. China

Zhuo Xiong

Key Laboratory for Advanced Manufacturing by Materials Processing Technology & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University, Beijing 100084, P.R. China

Feng Lin

Key Laboratory for Advanced Manufacturing by Materials Processing Technology & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University, Beijing 100084, P.R. China

Rendong Wu

Key Laboratory for Advanced Manufacturing by Materials Processing Technology & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University, Beijing 100084, P.R. China

Renji Zhang

Key Laboratory for Advanced Manufacturing by Materials Processing Technology & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University, Beijing 100084, P.R. China

There is a need for rapid fabrication of tissue or organs with well-defined structures and functions in regenerative medicine. Two patterns of cell/matrix constructs containing hepatic cells, gelatin and fibrinogen were successfully created by automated rapid prototyping techniques and stabilized with thrombin. No apparent cell damage was found during the process. Mechanical characterization demonstrated that a 1:1 ratio gelatin/fibrin mixture had the greatest elasticity modulus and compressive strength. Microscopic and histological observations showed that hepatic cells were embedded in the gelatin/fibrinogen matrix and were proliferating. Immunostaining and biochemical analysis indicated that the embedded hepatocytes secreted albumin. Fibrin appears to be a favorable component for a gelatin based cell assembly matrix in that it is bioresorbable, easily manipulated, and supports in vitro cell functions.

Key Words: rapid prototyping • three-dimensional constructs • hepatocytes • gelatin • fibrinogen • gelatin/fibrinogen matrix • scaffolds • tissue regeneration • tissue engineering.

Journal of Bioactive and Compatible Polymers, Vol. 22, No. 4, 363-377 (2007)
DOI: 10.1177/0883911507079451


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