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Biocompatible Fibroin Blended Films with Recombinant Human-like Collagen for Hepatic Tissue Engineering

Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China

Q. Lv

Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China

F. Z. Cui

Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China, cuifz{at}mail.tsinghua.edu.cn

Q. L. Feng

Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China

X. D. Kong

Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China

H. L. Wang

Beijing Institute of Biotechnology, Beijing 100071, P.R. China

L. Y. Huang

Beijing Institute of Biotechnology, Beijing 100071, P.R. China

T. Li

Beijing Institute of Biotechnology, Beijing 100071, P.R. China

Recombinant human-like collagen (RHLC) was blended with fibroin to prepare a novel biocompatible film as a scaffold material for hepatic tissue engineering applications. Solution blending was used to incorporate RHLC with silk fibroin to enhance the blend films biocompatibility and hydrophilicity while maintaining elasticity. FTIR and XRD analysis indicated that hydrogen bonds had formed between fibroin and RHLC, while SEM microscopy data confirmed that homogeneous microstructures were still retained after the introduction of RHLC with fibroin. Contact angle measurements indicated that the hydrophilicity of the fibroin/RHLC films was greater after RHLC was added. The elongation at break in the wet state was not markedly changed after blending the recombinant human-like collagen, which implied that flexibility was maintained. The proliferation and viability of the cell cultures on fibroin/RHLC films were significantly enhanced compared to pure fibroin films or tissue culture plates.

Key Words: fibroin • the recombinant human-like collagen • film • hepatocyte • tissue engineering

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