This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Suda, Y. Articles by Sobel, M. Search for Related Content Social Bookmarking                   What's this?

Effective Fluorescent and -Iodine Labelling of Proteins with a New Labelling Reagent

Department of Chemistry Faculty of Science Osaka University Toyonaka, Osaka 560, Japan

Shoichi Kusumoto

Department of Chemistry Faculty of Science Osaka University Toyonaka, Osaka 560, Japan

Raphael M. Ottenbrite

Department of Chemistry Virginia Commonwealth University Richmond, VA 23284

Michael Sobel

Department of Surgery Virginia Commonwealth University Richmond, VA 23249

A new fluorescent labelling reagent, which was previously devel oped for the labelling of polysaccharides, was also applied to the labelling of non-glycosilated proteins, aspartate aminotransferase and tumor necrosis factor-. Both proteins were labelled over a range of fluorescent substitutions (0.2 to 1.6 fluorescent moieties per protein molecule) without loss of biological activity. The fluorescent reagent can also be initially labelled with ¹²⁵-iodine. The specific radioactivity and biologic activity of labelled tumor necrosis factor- were equivalent to that prepared by a conventional technique for radio iodination. It is demonstrated that this new derivative chemistry may be ap plicable to the non-destructive labelling of a wide range of proteins in addition to polysaccharides.

Journal of Bioactive and Compatible Polymers, Vol. 9, No. 3, 310-317 (1994)
DOI: 10.1177/088391159400900305


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?