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Journal of Bioactive and Compatible Polymers
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Extracellular K5 Polysaccharide of Escherichia coli: Production and Characterization

Matilde Manzoni

Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche Sezione di Microbiologia Industriale Università degli Studi Via Celoria 2, 20133 Milano, Italy

Silvia Bergomi

Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche Sezione di Microbiologia Industriale Università degli Studi Via Celoria 2, 20133 Milano, Italy

Valeria Cavazzoni

Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche Sezione di Microbiologia Industriale Università degli Studi Via Celoria 2, 20133 Milano, Italy

The extracellular form of the K5 polysaccharide was produced, purified and characterized from a strain of Escherichia coli, the maximum fermentation yield (320 mg/L) was obtained after 15 h. The polysaccharide, recovered by precipitation with cold ethanol (80%), was purified through a pro cess employing an enzymic deproteination step using fungal protease. Charac terization by TLC and GC chromatography revealed that the K5 was mainly composed of glucuronic acid and N-acetyl-glucosamine. The 13C-NMR spectrum showed the product's structure to be the same as that of the K5 capsular polysaccharide described in the literature.

Journal of Bioactive and Compatible Polymers, Vol. 8, No. 3, 251-257 (1993)
DOI: 10.1177/088391159300800304
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