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Journal of Bioactive and Compatible Polymers
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Investigation of Dynamic Behaviors of Asialoglycoprotein Receptors of Hepatocytes Using a Latex Probe Whose Surface Was Coated with Lactose-Carrying Polystyrene

Takeshi Kugumiya

Faculty of Technology, Tokyo University of Agriculture and Technology Koganei, Tokyo 184, Japan

Akio Yagawa

Faculty of Technology, Tokyo University of Agriculture and Technology Koganei, Tokyo 184, Japan

Atsushi Maeda

Faculty of Technology, Tokyo University of Agriculture and Technology Koganei, Tokyo 184, Japan

Hirokazu Nomoto

Faculty of Technology, Tokyo University of Agriculture and Technology Koganei, Tokyo 184, Japan

Seishiro Tobe

Kanagawa Academy of Science and Technology Takatsu, Kawasaki 213, Japan

Kazukiyo Kobayashi

Faculty of Agriculture, Nagoya University Chikusa, Nagoya 464-01, Japan

Takehisa Matsuda

National Cardiovascular Center Suita, Osaka 564, Japan

Toshimasa Onishi

Nagase Chemicals Ltd., Tatsuno, Hyougo 679-41, Japan

Toshihiro Akaike

Faculty of Bioscience and Technology, Tokyo Institute of Technology Midori, Yokohama 227, Japan

Characteristic properties of oligosaccharide-substituted poly styrenes as a hepatocyte-specific culture substratum were investigated from the viewpoint of adsorption and recognition. X-ray photoelectron spectra sug gest that the adsorption of the polymer onto a polystyrene film occurred in near mono-molecular layer thickness of 10-25 Å in globular conformations. Distri bution and migration of asialoglycoprotein receptors on the surface of a hepato cyte was followed under a scanning electron microscope using a latex probe whose surface was coated with lactose-substituted polystyrene.

Journal of Bioactive and Compatible Polymers, Vol. 7, No. 4, 337-345 (1992)
DOI: 10.1177/088391159200700403
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