This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Rashid, S. N. Articles by Mikat, E. M. Search for Related Content Social Bookmarking                         What's this?

The Effect of Interstitial Air on the In Vitro Thrombogenicity of ePTFE Vascular Grafts

Department of Biomedical Engineering Duke University Durham, NC 27707

Howard G. Clark

Department of Biomedical Engineering Duke University Durham, NC 27707

Richard D. Vann

Department of Anesthesiology and F.G. Hall Hyperbaric Center Duke University Durham, NC 27707

Wayne A. Gerth

Department of Anesthesiology and F.G. Hall Hyperbaric Center Duke University Durham, NC 27707

Lirio A. Palmos

Department of Pathology Duke University Durham, NC 27707

Eileen M. Mikat

Department of Pathology Duke University Durham, NC 27707

Gas trapped in the interstices of the biomaterials used for vascular prostheses causes thrombosis, and the process of eliminating this gas is known as denucleation. An apparatus was developed for testing the in vitro effects of denucleation on 4 mm I.D. expanded polytetrafluoroethylene (ePTFE) Vitagraft (Johnson and Johnson). The apparatus was designed to ensure that neither the blood nor the grafts came in contact with air. Blood from a single donor was incubated with control and denucleated grafts for 5, 10, 15, 20, and 30 minutes. The thrombus volume in the graft lumen was measured with a computer assisted videometric system. Little thrombus formed by 5 or 10 minutes, but there was less thrombus in the denucleated graft than in the con trol graft at all times. The differences were statistically significant at 15 and 20 minutes (p < 0.05). Denucleation nearly doubled the thrombus formation time. Thrombus was more adherent to denucleated grafts than to control grafts. These results are consistent with in vivo observations in the rat where denucleation decreased thrombus formation and increased patency duration.

Journal of Bioactive and Compatible Polymers, Vol. 7, No. 1, 54-64 (1992)
DOI: 10.1177/088391159200700106


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?