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Journal of Bioactive and Compatible Polymers
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Immunogenicity of Protein-N-(2-Hydroxypropyl)methacrylamide Copolymer Conjugates in A/J and B10 Mice

P.A. Flanagan

Cancer Research Campaign Laboratory Department of Biological Sciences University of Keele Keele, Staffs. ST5 5BG, England

R. Duncan

Cancer Research Campaign Laboratory Department of Biological Sciences University of Keele Keele, Staffs. ST5 5BG, England

B. Rihova

Institute of Microbiology Czechoslovak Academy of Sciences Videnska 1083, 142 20 Prague 4

V. SUbr

Institute of Macromolecular Chemistry Czechoslovak Academy of Sciences 162 06 Prague 6, Czechoslovakia

J. Kopecek

Institute of Macromolecular Chemistry Czechoslovak Academy of Sciences 162 06 Prague 6, Czechoslovakia

Two proteins (model targeting residues) human immunoglobulin fraction (IgG) and human transferrin have been conjugated to N-(2-hydroxy propyl)methacrylamide (HPMA) copolymer and the antibody titer elicited, after subcutaneous or intraperitoneal administration to A/J and B10 mice of free and conjugated protein, was measured using the ELISA technique. The measured IgG titer against protein-HPMA copolymer conjugates was always higher than the IgM titer. Also, the titer (IgG) measured against native protein was up to 250-fold greater than that raised against protein-HPMA copolymer conjugates. This reduction in antibody titer against conjugate had a limited de pendence on its molecular weight.

Key Words: immunogenicity • N-(2-hydroxypropyl)methacrylamide copoly mers • transferrin • immunoglobulin IgG • drug-carrier.

Journal of Bioactive and Compatible Polymers, Vol. 5, No. 2, 151-166 (1990)
DOI: 10.1177/088391159000500201
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