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Journal of Bioactive and Compatible Polymers
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Polysaccharide-Coated Immunoliposomes Bearing Anti-CEA Fab' Fragment and Their Internalization by CEA-Producing Tumor Cells

Toshinori Sato

Laboratory of Artificial Cell Technology Department of Industrial Chemistry Faculty of Engineering Nagasaki University Nagasaki, 852 Japan

Junzo Sunamoto

Laboratory of Artificial Cell Technology Department of Industrial Chemistry Faculty of Engineering Nagasaki University Nagasaki, 852 Japan

Nobuko Ishii

Laboratory of Artificial Cell Technology Department of Industrial Chemistry Faculty of Engineering Nagasaki University Nagasaki, 852 Japan

Toshihiko Koji

Laboratory of Artificial Cell Technology Department of Industrial Chemistry Faculty of Engineering Nagasaki University Nagasaki, 852 Japan

Immunoliposomes, bearing Fab' fragments of anti-carcinoembryonic antigen (anti-CEA) monoclonal antibody, have been prepared according to a new method developed in our laboratory. First, egg phosphatidylcholine liposomes were coated with pullulan derivatives. Then, SH-bearing Fab' fragments of anti-CEA were conjugated to the pullulan derivatives attached to the liposomal surface. These immunoliposomes showed specific binding to CEA-producing cells, BM314, which was approximately 20-fold greater than to CEA-non- producing cells, RPMI #4788. In addition, these immunoliposomes were endo cytosed by colon cancer cells, which was confirmed by fluorescence microscopy using liposomes labelled by a hydrophobic fluorescent probe, terbium trisace tylacetonate.

Journal of Bioactive and Compatible Polymers, Vol. 3, No. 3, 195-204 (1988)
DOI: 10.1177/088391158800300301
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