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Effects of Chitosan on Properties of Novel Human-like Collagen/Chitosan Hybrid Vascular Scaffold

Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Taibai North Road 229 Xi'an, Shaanxi 710069, China

Daidi Fan

Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Taibai North Road 229 Xi'an, Shaanxi 710069, China, fandaidi{at}nwu.edu.cn

Xiaoxuan Ma

Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Taibai North Road 229 Xi'an, Shaanxi 710069, China

Wenjiao Xue

Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Taibai North Road 229 Xi'an, Shaanxi 710069, China

Yuanyuan Yu

Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Taibai North Road 229 Xi'an, Shaanxi 710069, China

Yane Luo

Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229, Xi'an, Shaanxi 710069, China, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Taibai North Road 229 Xi'an, Shaanxi 710069, China

Bowen Liu

Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Taibai North Road 229 Xi'an, Shaanxi 710069, China

Lan Chen

Faculty of Life Science, Northwest University Taibai North Road 229, Xi'an, Shaanxi 710069, China

Novel human-like collagen (HLC)/chitosan hybrid scaffolds were fabricated at blend ratios of 0%, 0.02%, 0.2% by crosslinking and freeze-drying process. The properties of the scaffolds were investigated, including morphology, mechanical strength, degradability, and cell biocompatibility. When the blend ratio was 0.02%, the morphology of the scaffolds was highly homogeneous with interconnected porous structure 46 ± 9 µm in size (SEM). The X-ray photoelectron spectroscopy analysis indicated intermolecular crosslinks between HLC and chitosan. The strain and stress of the scaffolds were 37.9 ± 3.3% and 309.7 ± 19.7 KPa, respectively. Human venous fibroblasts were expanded and seeded into the scaffolds in the density of 1 x 10 ⁵ cells/cm³ under static conditions. The cell morphology and proliferation were investigated using SEM, H&E, and MTT assay, which showed that the optimal content of the chitosan was signifcantly enhanced the cells adhesion, proliferation, and viability, compared to pure HLC, pure chitosan, and 0.2% chitosan/HLC scaffolds. These hybrid scaffolds appear to have favorable characteristics for vascular tissue engineering application.

Key Words: human-like collagen • chitosan • vascular scaffold • biocompatibility.

Journal of Bioactive and Compatible Polymers, Vol. 24, No. 6, 560-576 (2009)
DOI: 10.1177/0883911509349689


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