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Journal of Bioactive and Compatible Polymers
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Heparinized Micropatterned Surfaces for the Spatial Control of Human Mesenchymal Stem Cells

Jin Woo Bae

Department of Molecular Science and Technology, Ajou University 5 Woncheon, Yeongtong, Suwon 443-749, Republic of Korea

Jong Hoon Choi

Department of Molecular Science and Technology, Ajou University 5 Woncheon, Yeongtong, Suwon 443-749, Republic of Korea

Tae Eun Kim

Department of Molecular Science and Technology, Ajou University 5 Woncheon, Yeongtong, Suwon 443-749, Republic of Korea

Ki Dong Park

Department of Molecular Science and Technology, Ajou University 5 Woncheon, Yeongtong, Suwon 443-749, Republic of Korea, kdp{at}ajou.ac.kr

Ji Youn Kim

Department of Biomedical Engineering Korea University Medical College, Seoul, Republic of Korea

Yong Doo Park

Department of Biomedical Engineering Korea University Medical College, Seoul, Republic of Korea

Kyung Sun

Department of Biomedical Engineering Korea University Medical College, Seoul, Republic of Korea

In this study, a heparinized micropattern surface was prepared for the spatial control of human mesenchymal stem cells (hMSCs) that can differentiate into the desired tissues. Poly(styrene-co-vinylbenzyl N,N-diethyl-dithiocarbamate) (poly(ST-co-VBDC)) was synthesized as a photoreactive polymer; poly(ethylene glycol) methacrylate (PEGMA) was polymerized on the poly(ST-co-VBDC) coated surface by UV irradiation. XPS spectra revealed the residual DC moieties on the PEGMA-grafted surface and the linear chain growth of PEGMA was monitored according to irradiation time. After chemical immobilization of heparin onto this PEGMA surface, surface micropatterning was carried out by additional photopolymerization of PEGMA using a photomask. After incubation for 4 hour, the hMSCs adhered to the heparinized surface, while the hydrophilic PEGMA surface demonstrated no cell adhesion even after basic fibroblast growth factor (bFGF) treatment. Good alignment of hMSCs on the pattern-surface was distinctly observed along micron-sized grooves due to the presence of both heparin and bFGF. This heparinized micropattern surface can be used to study in vitro hMSCs responses with various heparin-binding growth factors in tissue engineering fields as well as cellular array for the spatial control of hMSCs.

Key Words: surface-micropatterning cells • heparin • PEGMA photograft • human mesenchymal stem cells • basic fibroblast growth factor • tissue engineering.

Journal of Bioactive and Compatible Polymers, Vol. 24, No. 6, 493-506 (2009)
DOI: 10.1177/0883911509349143
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