Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Sign In to gain access to subscriptions and/or personal tools.
Journal of Bioactive and Compatible Polymers
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Wu, M.-Y.
Right arrow Articles by Chen, S.-H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Chitosan/Alginate Multilayer Scaffold Encapsulating Bone Marrow Stromal Cells In Situ on Titanium

Ming-Yue Wu

College of Stomatology, Nanjing Medical University, Nanjing, China

Ning Chen

College of Stomatology, Nanjing Medical University, Nanjing, China, wumingyue321{at}126.com

Lai-Kui Liu

College of Stomatology, Nanjing Medical University, Nanjing, China

Hua Yuan

College of Stomatology, Nanjing Medical University, Nanjing, China

Quan-Li Li

College of Stomatology, Anhui Medical University, Hefei, China

Shou-Hui Chen

Department of Medical Biotechnology Anhui Medical University, Hefei, China

A biofilm-like scaffold with bone marrow stromal cells (BMSC) encapsulated in situ was constructed on a titanium surface using a layer-by-layer self-assembly technique for the potential application for dental or joint implant. The scaffold was formed by depositing a single layer of positively charged poly(L-lysine) on a negatively charged NaOH-treated titanium substrate, followed by alternate immersion into a negatively charged alginate—BMSC suspension and positively charged chitosan solution, respectively, and terminated a layer of chitosan. The cell-encapsulated scaffolds were evaluated by scanning electron microscopy and confocal laser scanning microscopy. The BMSC remained viable and grew well in the scaffold. This approach provides a method for the preparation of tissue engineering scaffold on titanium surfaces.

Key Words: titanium • tissue engineering • layer-by-layer assembly • alginate • chitosan.

Journal of Bioactive and Compatible Polymers, Vol. 24, No. 4, 301-315 (2009)
DOI: 10.1177/0883911509105848
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?