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Hyaluronic Acid Hydrogel Modified with Nogo-66 Receptor Antibody and Poly(L-Lysine) Enhancement of Adherence and Survival of Primary Hippocampal Neurons

Laboratory of Advanced Materials, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China

Xiao-Dan Sun

Laboratory of Advanced Materials, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China

Xue Xia

Laboratory of Advanced Materials, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China

Fu-Zhai Cui

Laboratory of Advanced Materials, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. China, cuifz{at}mail.tsinghua.edu.cn

Yu He

Beijing Institute for Neuroscience, Beijing Center for Neural Regeneration and Repairing, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Capital Medical University, Beijing 100069, P.R. China

Bing-Fang Liu

Beijing Institute for Neuroscience, Beijing Center for Neural Regeneration and Repairing, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Capital Medical University, Beijing 100069, P.R. China

Qun-Yuan Xu

Beijing Institute for Neuroscience, Beijing Center for Neural Regeneration and Repairing, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Capital Medical University, Beijing 100069, P.R. China

Hyaluronic acid (HA) hydrogel was modified with poly(L-lysine) (PLL) and Nogo-66 Receptor antibody (antiNgR) to enhance the repair of central nervous system (CNS) injuries. The immobilization of PLL was characterized by X-ray photoelectron spectroscopy (XPS) and the immobilization of antiNgR was studied by immunofluorescence. The cytocompatibility of this modified hydrogel was analyzed by culturing primary hippocampal neurons. The quantity and morphology of the neurons were influenced by different modifications; the primary hippocampal neurons cultured with modified HA hydrogel exhibited multipolar and bipolar morphology were compared with unmodified hydrogel cultures. The number of neurons obtained by culturing with HA hydrogel modified with both PLL and antiNgR was almost twice the number of neurons cultured with HA modified with only PLL or antiNgR. This phenomenon was attributed to the collaborative effect of PLL and antiNgR on the neurons. The characteristics of this new hydrogel system, including pore structure, water absorption, hydrolysis degradation did not change much when compared with the hydrogel modified with PLL or antiNgR, respectively. It is expected that this modified HA hydrogel has potential as a CNS tissue engineering material.

Key Words: hyaluronic acid • hydrogel • antiNgR • poly(L-lysine) • cell attachment • tissue engineering • biomaterial • bioengineering • central nervous system injuries • repairing CNS injuries • modified hyaluronic acid hydrogels.

Journal of Bioactive and Compatible Polymers, Vol. 24, No. 3, 205-219 (2009)
DOI: 10.1177/0883911509102266


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