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Journal of Bioactive and Compatible Polymers
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Design and Evaluation of a Cell Microencapsulating Device for Cell Assembly Technology

Rui Yao

Key Laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering, Tsinghua University Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University Beijing 100084, P.R. China

Renji Zhang

Key Laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering, Tsinghua University Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University Beijing 100084, P.R. China

Xiaohong Wang

Key Laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering, Tsinghua University Beijing 100084, P.R. China, Institute of Life Science & Medicine, Tsinghua University Beijing 100084, P.R. China, wangxiaohong{at}tsinghua.edu.cn

A cell encapsulation device based on the principle of high-voltage dispersion technology was designed and constructed. The parameters that influenced the formation of cell microcapsules, such as; voltage, push speed, electrode distance, and syringe size were analyzed and optimized. Unlike the traditional cell microencapsulating devices, both of the electrodes were separated from the CaCl2 solution in this device, which simplified the procedure of keeping the operation within a sanitary environment. Integrated adipose derived stem cell (ADSC) microcapsules with smooth surface and consistent diameter distribution were prepared. After 1-week in culture as a microcapsule and 3 days assembled in a construct, the ADSCs maintained a high viability and significant proliferation that established a favorable basis for the controlled assembly of cell microcapsules.

Key Words: controlled assembly • high-voltage dispersion technology • microencapsulation • adipose derived stem cells (ADSCs) • tissue engineering.

Journal of Bioactive and Compatible Polymers, Vol. 24, No. 1 Suppl, 48-62 (2009)
DOI: 10.1177/0883911509103329
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