This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Xu, M. Articles by Wang, X. Search for Related Content Social Bookmarking                         What's this?

Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique

Key laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering, Tsinghua University Beijing 100084, P.R. China; Institute of Life Science & Medicine, Tsinghua University Beijing 100084, P.R. China; Center Laboratory of Tissue Engineering, Hangzhou Dianzi University, Hangzhou 310018, P.R. China, xumingen{at}tsinghua.edu.cn

Yongnian Yan

Key laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering, Tsinghua University Beijing 100084, P.R. China; Institute of Life Science & Medicine, Tsinghua University Beijing 100084, P.R. China

Haixia Liu

Key laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering, Tsinghua University Beijing 100084, P.R. China; Institute of Life Science & Medicine, Tsinghua University Beijing 100084, P.R. China

Rui Yao

Key laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering, Tsinghua University Beijing 100084, P.R. China; Institute of Life Science & Medicine, Tsinghua University Beijing 100084, P.R. China

Xiaohong Wang

Key laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering, Tsinghua University Beijing 100084, P.R. China; Institute of Life Science & Medicine, Tsinghua University Beijing 100084, P.R. China, wangxiaohong{at}tsinghua.edu.cn

One of the major obstacles in engineering thick and complex tissues while vascularizing tissues in vitro is to maintain cell viability during tissue growth and structural organization. Adipose-derived stromal (ADS) cells were used to establish a multicellular system through a cell-assembly technique. Attempts were made to control ADS cells differentiation into different targeted cell types according to their positions within an orderly 3D structure. Oil red O staining confirmed that the ADS cells in the structure differentiated into adipocytes with a spherical shape while immunostaining tests confirmed that the endothelial growth factor induced ADS cells on the walls of channels differentiated into mature endothelial cells and then organized into tubular structures throughout the engineered 3D structure. The endothelin-1 and nitric oxide release rules of the endothelial cells were coincidental with those in vivo. This study provides a new approach to engineer orderly endothelial vessel networks in vitro and has potential applications in adipose-tissue engineering.

Key Words: adipose-derived stromal (ADS) cells • cell-assembly technique • vascularize • endothelial vessel network • adipose-tissue engineering • tissue engineering.

Journal of Bioactive and Compatible Polymers, Vol. 24, No. 1 Suppl, 31-47 (2009)
DOI: 10.1177/0883911509102794


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?