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In Vitro Cytotoxicity of Bacterial Cellulose Scaffolds Used for Tissue-engineered Bone

School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P.R. China

Tingfei Xi

School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P.R. China, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, P.R. China, Shenzhen Institute, Peking University, Shenzhen 518057, P.R. China, xitingfei{at}tom.com

Yudong Zheng

School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P.R. China

Tingting Guo

College of Medicine Laboratory, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, PR. China

Jiaquan Hou

Building Design and Research Institute of the General Logistics Department of PLA, Beijing 100036, PR. China

Yizao Wan

School of Materials Science and Engineering Tianjin University, Tianjin 300072, PR China

Chuan Gao

School of Materials Science and Engineering Tianjin University, Tianjin 300072, PR China

The in vitro degradation and cytotoxicity of bacterial cellulose (BC) and its degradation products were studied for potential applications in bone tissue engineering. Emission scanning electron microscope was used to observe the morphology of original materials and their degradation products. The degradation was evaluated by measuring the concentration of reducing sugar by using ultraviolet spectrophotometer. Bone forming osteoblast (OB) cells and infinite culture cell line L929 fibroblasts were used to measure the cytotoxicity of materials using the MTT assay. Both types of cells proliferated normally with the BC and its degradation products with a cytotoxicity graded of 0—1. Nevertheless, the bone-forming target OB cells were more susceptible to cytotoxicity than the infinite culture fibroblast cells L929 fibroblasts. The results indicate that the BC is not very cytotoxic and that tissue functional cells are more suitable for evaluating the cytotoxicity of biomedical materials.

Key Words: bacterial cellulose (BC) • osteoblast • degradability • biocompatibility • bone tissue engineering • tissue engineering.

Journal of Bioactive and Compatible Polymers, Vol. 24, No. 1 Suppl, 137-145 (2009)
DOI: 10.1177/0883911509102710


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