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In Vitro Angiogenesis of 3D Tissue Engineered Adipose Tissue

Key Laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine Tsinghua University, Beijing 100084, P.R. China

Renji Zhang

Key Laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine Tsinghua University, Beijing 100084, P.R. China

Yongnian Yan

Key Laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine Tsinghua University, Beijing 100084, P.R. China

Xiaohong Wang

Key Laboratory for Advanced Materials Processing Technology Ministry of Education & Center of Organ Manufacturing Department of Mechanical Engineering Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science & Medicine Tsinghua University, Beijing 100084, P.R. China, wangxiaohong{at}tsinghua.edu.cn

Using a cell assembly device developed in this laboratory, we coextruded adipose derived stem cells (ADSC) and gelatin/alginate hydrogel to form cubic 3D constructions (10 x 10 x 10 mm³) with regular distributed go-through pores. The ADSC grew, proliferated, and differentiated within these constructions. With the addition of basic fibroblast growth factor (bFGF), cells located on the scaffold walls differentiated into endothelial like cells while cells embedded in the hydrogel differentiated into adipose like cells. The integrity of the constructions remained for more than 60 days. This new technology enables us to construct 3D adipose tissue with blood vessel-like structures in vitro which is a significant enhancement in adipose tissue engineering and provides a better biomimic 3D model for studying cell—cell interaction, stem cell differentiation conditions and cell organization mechanisms.

Key Words: 3D adipose tissue • cell assembly • blood vessel-like structures • adipose derived stem cells (ADSC) • tissue engineering • mimetic blood vessels.

Journal of Bioactive and Compatible Polymers, Vol. 24, No. 1, 5-24 (2009)
DOI: 10.1177/0883911508099367


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