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Three-dimensional Cultures of Rat Pancreatic RIN-5F Cells in Porous PLGA-collagen Hybrid Scaffolds

Biomaterials Center, National Institute for Materials Science 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

Xiaoting Lin

Biomaterials Center, National Institute for Materials Science 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

Tetsuya Tateishi

Biomaterials Center, National Institute for Materials Science 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

Guoping Chen

Biomaterials Center, National Institute for Materials Science 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan, guoping.chen{at}nims.go.jp

Three-dimensional cultures of pancreatic islet cells in porous scaffolds or hydrogels have been constructed as a biohybrid artificial pancreas. A thin mesh of a PLGA-collagen hybrid was used to culture rat RIN-5F cells. The hybrid mesh was coated with laminin, fibronectin, vitronectin, type IV collagen, and poly(L-lysine) were evaluated and mesh without coating was used as a control. Cell adhered and proliferated on all of the coated and uncoated meshes. The cells formed spheroids in the uncoated, poly(L-lysine)-, fibronectin-, vitronectin-, and type IV collagen-coated hybrid meshes, while forming a layered structure in the laminin-coated hybrid mesh. Cell adhesion on the coated PLGA-collagen hybrid meshes was higher than that for the uncoated hybrid mesh. The laminin-coated hybrid mesh showed the greatest level of adhesion. The insulin secretion capacity of RIN-5F cells was at the same level for all coated and uncoated PLGA-collagen hybrid meshes and higher than that of cells cultured on cell culture plates. The 3D cultured PLGA-collagen hybrid meshes promoted insulin production capacity. Gene expression analysis showed that genes encoding insulin I, insulin II, and the pancreatic transcription factor PDX-1 (pancreas/duodenum homeobox 1) was expressed. These results indicate that the PLGA-collagen hybrid meshes support adhesion, proliferation, and differentiation of RIN-5F cells that allows culturing pancreatic islet cells on 3D constructs.

Key Words: tissue engineering • pancreatic islet • porous scaffold • 3D culture • PLGA-collagen hybrid mesh.

Journal of Bioactive and Compatible Polymers, Vol. 24, No. 1, 25-42 (2009)
DOI: 10.1177/0883911508099439


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