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Bioactivity of a Novel Nano— composite of Hydroxyapatite and Chitosan—Phosphorylated Chitosan Polyelectrolyte Complex

Faculty of Stomatology, Anhui Medical University, Hefei, China, ql-li{at}126.com

Ming-Yue Wu

Faculty of Stomatology, Anhui Medical University, Hefei, China

Li-Li Tang

Faculty of Stomatology, Anhui Medical University, Hefei, China

Jian Zhou

Faculty of Stomatology, Anhui Medical University, Hefei, China

Yong Jiang

Faculty of Stomatology, Anhui Medical University, Hefei, China

Brian W. Darvell

Dental Materials Science, Faculty of Dentistry, The University of Hong Kong, Hong Kong

The bioactivity of a novel composite of carbonate-containing low-crystallinity nanoparticle hydroxyapatite (HA) and a chitosan—phosphorylated chitosan polyelectrolyte complex (PEC) was evaluated in vitro and in vivo. The HA—PEC nanocomposite with complicated porous structure was prepared by a biomimetic method. An acidic chitosan (polycation) solution containing calcium and phosphate ions (6 mM Ca²⁺, Ca/P: 1.67) was added into phosphorylated chitosan (polyanion) solution; the formation of PEC and the controlled HA crystal growth were co-organized in alkaline solution. The material was co-cultured with rat osteoblasts in vitro, and implanted into rabbit femur marrow cavities. The results indicate that the PEC—HA composite promoted osteoblast adhesion, morphology, proliferation, and differentiation in vitro; the bone tissue response to the material histologically showed that it was bioactive, as well as biodegradable. The HA—PEC composite shows promise as a bone-repair material.

Key Words: Chitosan • phosphorylated chitosan • polyelectrolyte complexes • hydroxyapatite • in vitro proliferation and differentiation • in vivo activity • osteoblast adhesion • biomimesis.

Journal of Bioactive and Compatible Polymers, Vol. 23, No. 6, 520-531 (2008)
DOI: 10.1177/0883911508097370


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