Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Sign In to gain access to subscriptions and/or personal tools.
Journal of Bioactive and Compatible Polymers
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via ISI Web of Science (6)
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Dandu, R.
Right arrow Articles by Cappello, J.
Right arrow Search for Related Content
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Characterization of Structurally Related Adenovirus-laden Silk-elastinlike Hydrogels

Ramesh Dandu

Center for Nanomedicine & Cellular Delivery, Department of Pharmaceutical Sciences, University of Maryland, Baltimore, Baltimore, MD, USA

Hamidreza Ghandehari

Center for Nanomedicine & Cellular Delivery, Department of Pharmaceutical Sciences, University of Maryland, Baltimore, Baltimore, MD, USA, hghandeh{at}rx.umaryland.edu

Joseph Cappello

Protein Polymer Technologies, Inc., San Diego, CA, USA

Genetically engineered silk-elastinlike protein polymer (SELP) analogs, SELP-47 K and -415 K, consisting of varying ratio(s) and length(s) of silk and elastin in their monomer repeats and capable of spontaneous hydrogel formation were evaluated as matrices for controlled adenoviral release in vitro. The degree of swelling (q) and the amount of soluble fraction of SELP hydrogel analogs were evaluated with and without the incorporation of adenoviruses. The results indicate that polymer concentration and structure and not the incorporated adenoviruses are the predominant factors that influence q and the soluble fraction in these hydrogels over a 28-day period. The release of adenoviruses was a function of polymer concentration and structure. The higher cumulative percentage release observed in SELP-415 K compared to SELP-47 K can be attributed to the longer elastin sequence in the polymer backbone. These results indicate the potential of customizing the network properties and release from SELPs by manipulating the macromolecular architecture using recombinant synthesis.

Key Words: adenoviral gene delivery • controlled gene delivery • genetically engineered polymers • hydrogels • silk-elastinlike protein polymers • soluble fraction and swelling.

Journal of Bioactive and Compatible Polymers, Vol. 23, No. 1, 5-19 (2008)
DOI: 10.1177/0883911507085278
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?