Journal of Bioactive and Compatible Polymers

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to register today!

Click here to register today!

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrow Add to My Marked Citations
Google Scholar
Right arrow Articles by Chognot, D.
Right arrow Articles by Vigneron, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Bioactive and Compatible Polymers, Vol. 23, No. 1, 49-68 (2008)
DOI: 10.1177/0883911507085280
© 2008 SAGE Publications

Synthesis and In vivo Studies of Protein C-loaded Nanoparticles with PEO Modified Surfaces

David Chognot

Laboratoire de Chimie Physique Macromoléculaire, UMR CNRS-INPL 7568 ENSIC, BP 20451, 54001 Nancy Cedex, France

Jean-Luc Six

Laboratoire de Chimie Physique Macromoléculaire, UMR CNRS-INPL 7568 ENSIC, BP 20451, 54001 Nancy Cedex, France

Michèle Léonard

Laboratoire de Chimie Physique Macromoléculaire, UMR CNRS-INPL 7568 ENSIC, BP 20451, 54001 Nancy Cedex, France

Edith Dellacherie

Laboratoire de Chimie Physique Macromoléculaire, UMR CNRS-INPL 7568 ENSIC, BP 20451, 54001 Nancy Cedex, France, edith.dellacherie@ ensic.inpl-nancy.fr

Béatrice Faivre

Mise en forme et évaluation de matériaux d'intérêt thérapeutique et biologique, EA 3452, Faculté de Pharmacie, BP 403, 54001 NANCY Cedex, France

François Bonneaux

Mise en forme et évaluation de matériaux d'intérêt thérapeutique et biologique, EA 3452, Faculté de Pharmacie, BP 403, 54001 NANCY Cedex, France

Claude Vigneron

Mise en forme et évaluation de matériaux d'intérêt thérapeutique et biologique, EA 3452, Faculté de Pharmacie, BP 403, 54001 NANCY Cedex, France

Protein C-loaded nanoparticles coated with monomethoxypoly (ethylene oxide) (MPEO) were prepared by double emulsion/solvent evaporation using water-soluble biocompatible copolymers of MPEO and polylactide, as surfactants of the secondary emulsion. The nanoparticle preparation was optimized to obtain the best yield of encapsulated protein C and provide the greatest retention of its biological activity. The nanoparticles were characterized in terms of size, zeta potential, and thickness of the MPEO external layer. Protein C-loaded nanoparticles were injected into the bloodstream of guinea pigs and the protein concentration in plasma is measured as a function of time. After a rapid release corresponding to 20% of the injected protein, the protein plasma concentration progressively decreased and reached a value close to zero after 5 h. Consequently, the in vivo fate of the fluorescent nanoparticles coated with or without MPEO is studied. The uncoated nanoparticles were rapidly captured by the circulating granulocytes while the coated ones were not. The histological analysis of the spleen, 1 hour after injection, showed that the MPEO-coated particles were retained in this organ, while the uncoated ones were not captured.

Key Words: encapsulation • protein C • MPEO-coated nanoparticles • experiments on guinea pigs • spleen filtration.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?