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Chitosan/Carboxymethyl Cellulose Polyelectrolyte Complex Scaffolds for Pulp Cells Regeneration

Key Laboratory for Oral Biomedical Engineering, Ministry of Education School of Stomatology, Wuhan University, Wuhan 430079, PR China

Mingwen Fan

Key Laboratory for Oral Biomedical Engineering, Ministry of Education School of Stomatology, Wuhan University, Wuhan 430079, PR China, chenhuangqin79{at}163.com

Novel polyelectrolyte complex (PEC) chitosan/carboxymethyl cellulose (CMC) scaffolds are prepared using a freeze-dry process. The microstructure is characterized by SEM and the average pore diameters and internal porosities are calculated. The morphology and distribution of pulp cells on these three-dimensional scaffolds are investigated by SEM and confocal laser scanning microscopy (CLSM). The expression of osteonectin (ON) and dentin sialophosphoprotein (DSPP) is detected by reverse transcription polymerase chain reaction (RT-PCR). The scaffolds are then implanted subcutaneously into BALB/c mice. The addition of CMC to chitosan decreases the pore diameter and increases internal porosity. The pulp cells co-cultured in these PEC scaffolds show improved adhesion, spreading, cell capacity, and three-dimensional configurations compared to pure chitosan scaffolds. Based on the data obtained, the chitosan/CMC complex provides an improved scaffold material for pulp tissue engineering.

Key Words: polyelectrolyte complexes • polyelectrolyte complex scaffolds • chitosan • chitosan scaffolds • carboxymethyl cellulose • tissue engineering • pulp tissue regeneration.

Journal of Bioactive and Compatible Polymers, Vol. 22, No. 5, 475-491 (2007)
DOI: 10.1177/0883911507081329


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