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A Novel Poly(L-lactide) (PLLA)/Fibroin Hybrid Scaffold to Promote Hepatocyte Viability and Decrease Macrophage Responses

Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China

Q. Lv

Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China

F.Z. Cui

Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China, cuifz{at}mail.tsinghua.edu.cn

L. Xu

Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China

Y.P. Jiao

Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China

Y. Wang

Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China

Q.L. Feng

Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China

H.L. Wang

Beijing Institute of Biotechnology, Beijing 100071, People's Republic of China

L.Y. Huang

Beijing Institute of Biotechnology, Beijing 100071, People's Republic of China

The purpose of this study was to evaluate the hepatocellular compatibility and assess the inflammatory response of a novel hybrid scaffold of poly(L-lactide) (PLLA) and fibroin. The hybrid scaffold was obtained by freezing and lyophilizing a blend of fibroin microspheres and PLLA solution. FTIR and scanning electron microscopy (SEM) analysis indicated that fibroin microspheres were on the surface of the hybrid scaffold. Compared to the PLLA scaffold, SEM and laser scanning confocal microscope (LSCM) analyses showed that the human hepatocellular carcinoma HepG2 cells had spread and proliferated much more in the hybrid scaffold. The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assays indicated a greater number of cells in this hybrid scaffold. Furthermore, a mouse RAW264.7 macrophages cell line was utilized to characterize and compare the mRNA profiles of TNF-alpha using real time-polymerase chain reaction (RT-PCR). The inflammatory response of the macrophages grown in the PLLA/fibroin scaffold rapidly declined compared to those in the PLLA scaffold and reached the level of cells grown in Dulbecco's Modified Eagle Medium (DMEM). The hepatocellular compatibility and lower level of inflammatory response makes the PLLA/fibroin scaffold a promising candidate for hepatic tissue engineering.

Key Words: PLLA • fibroin • hybrid scaffold • cellular viability • inflammatory.

Journal of Bioactive and Compatible Polymers, Vol. 22, No. 4, 395-410 (2007)
DOI: 10.1177/0883911507079893


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