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Journal of Bioactive and Compatible Polymers
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Improved Pharmacological Properties for Superoxide Dismutase Modified with Carboxymethycellulose

Amalia Dominguez

Aimara Valdivia

Julio Caballero

Reynaldo Villalonga

Enzyme Technology Group, Center for Biotechnological Studies, University of Matanzas, Autopista a Varadero Km 3 1/2, Matanzas, C.P. 44740, Cuba

Gregorio Martínez

Center for Evaluation and Biological Research, Institute of Pharmacy, Havana University, San Lazaro y L, Ciudad de La Habana, Cuba

Etienne H. Schacht

Polymer Materials Research Group, Department of Organic Chemistry, University of Gent, Krijgslaan 281, S-4, B-9000 Gent. Belgium; reynaldo.villalonga{at}umcc.cu

Superoxide dismutase (SOD) was chemically modified with carboxymethylcellulose through two different synthetic procedures: Reductive alkylation with the periodate-oxidized polymer (SOD-CMCox), and the formation of amide linkages through a carbodiimide catalyzed reaction (SODCMCedac). The SOD-CMCox and SOD-CMCedac conjugates contained about 1.8–1.2mol of polymer per mol of protein, and retained 68–78% of the initial catalytic activity, respectively. The glycosidated enzymes were more resistant to inactivation with H2O2 and their plasma half-life times were prolonged to 34.7h – 6.6h when compared with 4.8min for native SOD. The anti-inflammatory activity of the enzyme was 2–2.4 times increased after conjugation with the polymer.

Key Words: superoxide dismutase • carboxymethylcellulose • anti-inflammatory • modified enzyme • pharmacokinetics

Journal of Bioactive and Compatible Polymers, Vol. 20, No. 6, 557-570 (2005)
DOI: 10.1177/0883911505059068
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