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Journal of Bioactive and Compatible Polymers
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Osteoblast MC3T3-E1 Culture on a Fast-setting Carbonated Hydroxyapatite Bone-like Material

K. Y. Mao

L. B. Hao

P. F. Tang

Y. Wang

J. F. Wang

S. B. Lu

Department of Orthopedics, The Chinese PLA General Hospital, Beijing 100853, China

S. S. Liao

Department of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan; liao{at}den.hokudai.ac.jp

F. Z. Cui

Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China

Using ultra-fine grain technology, a fast-setting type of carbonated hydroxyapatite (CHA) material from Ca3(PO4)2·3H2O (ACP), CaCO3, Ca(H2PO4)2 (MCPA) and Na3PO4 was prepared. The ultra-fine particles (3 m) shortens the setting time, to form bone like material with 5.2wt% carbonation. Osteoblastic MC3T3-E1 cells were cultured with CHA material to observe cell adhesion, proliferation and differentiation. There were no obvious differences in adhesion and proliferation rate of MC3T3-E1 cells between the CHA and the control group. MC3T3-E1 cells on the surface of CHA observed by a scanning electron microscope (SEM). The alkaline phosphatase (ALP) activity of the cells on the CHA was higher than the control, and collagen I (COL 1) mRNA expressed on the CHA was also significantly higher than the control. This CHA bone material exhibited biocompatibility and osteo-conductive properties that are sought for bone repair and scaffolding for tissue engineering. *Author to whom correspondence should be addressed.

Key Words: carbonated hydroxyapatite • osteoblast • biocompatibility • bone repair • tissue engineering • tissue scaffold

Journal of Bioactive and Compatible Polymers, Vol. 20, No. 6, 541-555 (2005)
DOI: 10.1177/0883911505059040
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