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Journal of Bioactive and Compatible Polymers
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Suitable Materials for Soft Tissue Reconstruction: In Vitro Studies of Cell – Triblock Copolymer Interactions

Monica Mattioli-Belmonte

Istituto di Morfologia Umana Normale, Facoltà di Medicina e Chirurgia, Università Politecnica delle Marche, Ancona, Italy; belmonte{at}med.univpm.it

Graziella Biagini

Guendalina Lucarini

Laura Virgili

Francesca Gabbanelli

Silvana Amati

Istituto di Morfologia Umana Normale, Facoltà di Medicina e Chirurgia, Università Politecnica delle Marche, Ancona, Italy

Francesca Cecchet

Laboratoire Interdisciplinaire de Spectroscopie Electronique (LISE), Namur, Belgium

Ann-Christine Albertsson

Department of Fibre and Polymer Technology, Royal Institute of Technology, Stockholm, Sweden; aila{at}polymer.kth.se

Anna Finne

Natalia Andronova

Department of Fibre and Polymer Technology, Royal Institute of Technology, Stockholm, Sweden

Keratinocytes and fibroblasts have been grown onto a series of triblock copolymers based on 1,5-dioxepan-2-one (DXO) and L-lactide (LLA). The molar ratio of DXO and LLA were varied in the copolymers. This resulted in different degrees of hydrophilicity, which in turn influenced the cell growth. On these surfaces, the morphological appearance of the cells with their cell movements and growth were investigated by means of scanning electron microscopy, time-lapse videomicroscopy and immunohistochemistry. All results clearly showed that the keratinocytes and fibroblasts adhered best to the most hydrophilic copolymers. A majority of the keratinocytes seeded on the most hydrophilic copolymer also presented a polarized morphology indicating a migration tendency. The cell growth onto these materials are interesting since a possible application for these unique materials is as polymeric membranes for guided cutaneous and/or periodontal tissue generation.

Key Words: triblock copolymers • hydrophilicity • cell culture • time-lapse video microscopy • cell morphology • cell movement • cell growth • tissue engineering

Journal of Bioactive and Compatible Polymers, Vol. 20, No. 6, 509-526 (2005)
DOI: 10.1177/0883911505058608
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H. Chen and M. Fan
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Journal of Bioactive and Compatible Polymers, September 1, 2007; 22(5): 475 - 491.
[Abstract] [PDF]