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Bone in Growth in bFGF Versus BMP Combined with bFGF Saturated PLA-PEG-PLA: Experiment in Surgically Created Rabbit Mandibular Defects

Department of Oral and Maxillofacial Surgery, Tongji Stomatological Hospital affiliated to Tongji University, 399 Yanchang Road, 200072 Shanghai, China, guangyu{at}hotmail.com

Kamulegeya Adriane

Department of Oral and Maxillofacial Surgery, Tongji Stomatological Hospital affiliated to Tongji University, 399 Yanchang Road, 200072 Shanghai, China

Pan Ke Feng

Department of Oral and Maxillofacial Surgery, Tongji Stomatological Hospital affiliated to Tongji University, 399 Yanchang Road, 200072 Shanghai, China

A triblock polymer of LPLA and PEG 1000 and 3400 at 3% and 9% was formed by ring opening under nitrogen atmosphere at 120°C and then, in one group, bFGF was added, while in the other both bFGF and BMP were added, leaving one group as a control. Gross, radiographic, histological and scanning electronic microscope observations were done. With the help of motic images advanced 3.0 new bone formed, calculated as a percentage of total selected area and, using SAS6.12, results were analyzed. Statistical analysis between groups showed that, at 2 weeks, the PLA-PEG-PLA + bFGF + BMP group had the highest amount of new bone formation, as a percentage of new bone over total surface area, at 48% followed by the PLA-PEG-PLA + bFGF group at 39%, then the PLA-PEG-PLA=BMP group at 29%. The PLA-PEGPLA group trailed in at 18% (statistical significance, p <0.05=0.0001). After 4 weeks, the experimental groups B and D had almost equal new bone formation (62% and 64% respectively), but still significantly different from the control group and BMP2 group, with 43% and 38% respectively (p <0.05=0.0001). After 8 weeks, there was no difference in the amount of bone regeneration (p >0.05=0.87).

Within the groups, the control group exhibited steady incremental new bone formation at 2, 4 and 8 weeks, starting out at 18%, followed by 42% and lastly 65%. Interestingly this phenomenon also applied to group C (BMP2 group) but not to the B group (bFGF group). The differences at the respective consecutive time of examination were statistically significant (p <0.05=0.0001). However, in both experimental groups B and D there was statistically significant difference between new bone formed at 2 weeks compared to that at 4 weeks, but thereafter the increment was negligible.

Key Words: tissue engineering • bFGF • BMP • PLA-PEG-PLA • synthetic biomaterials • growth factors • bone tissue

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