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Release Behavior and Biological Activity of Recombinant Human Bone Morphogenetic Protein-2 from Porous PLGA Scaffold

Stomatology Department of the First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003 China, zhuhy71{at}yahoo.com.cn, zhuhy0319{at}hotmail.com

Qiuliang Wu

Stomatology Department of the First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003 China

Jianzhong Shentu

Clinical Pharmacology Laboratory of the First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003 China

Huiming Wang

Stomatology Department of the First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003 China

Yingqian Hu

Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310003 China

Kangjie Zhu

Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310003 China

Jianhua Liu

Stomatology Department of the First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003 China

The feasibility of using the poly-(DL-lactic-co-glycolic acid) (PLGA) scaffold as a carrier for controlled recombinant human bone morphogenetic protein-2 (rhBMP-2) delivery was assessed. PLGA rhBMP-2-loaded scaffolds were fabricated by emulsion polymerization and freeze-dried; a porous morphology was observed by scanning electron microscopy. The in vitro release of rhBMP-2 from the polymer was assessed using high performance liquid chromatography. An initial burst release of the incorporated rhBMP-2 was observed over the first 24h followed by an 80.6% sustained release for one month. The rhBMP-2-loaded scaffolds were implanted bilaterally into rat ectopic muscle pouches. The rhBMP-2 implanted animals showed bone formation submuscle space and osteoblasts located in the new bone region while none was seen in the control groups.

Key Words: poly(DL-lactic-co-glycolic acid) • recombinant human bone morphogenetic protein-2 • scaffold • sustained release • biological activity • growth factors

Journal of Bioactive and Compatible Polymers, Vol. 20, No. 3, 271-278 (2005)
DOI: 10.1177/0883911505053384


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