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Direct Construction of a Three-dimensional Structure with Cells and Hydrogel

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China

Xiaohong Wang

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China, wangxiaohong{at}tsinghua.edu.cn

Zhuo Xiong

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China

Haixia Liu

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China

Feng Liu

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China

Feng Lin

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China

Rendong Wu

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China

Renji Zhang

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China

Qingping Lu

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, Institute of Life Science and Medicine, Tsinghua University, Beijing 100084, P.R. China

An organ manufacturing technique was developed that enables the formation of cell/extracellular matrix (ECM) complexes for in vitro or in vivo growth. In this study, a three-dimensional (3D) structure composed of hepatocytes and gelatin/alginate hydrogel was made using a cell assembler-I apparatus to thoroughly control cell assembling. Hepatocytes and ECM were constructed into 10 X 10 X 3mm³ structures according to a designed pattern. The embedded hepatocytes remained viable and performed biological functions in the construct for more than 12 days. This 3D structure has the potential to be used as a precursor for tissue or organ regeneration. This technology offers the potential for high-throughput production of artificial human tissues and organs.

Key Words: cell assembly • rapid prototyping • three-dimensional precursor • implantable bioartificial liver • hydrogel • hepatocyte

Journal of Bioactive and Compatible Polymers, Vol. 20, No. 3, 259-269 (2005)
DOI: 10.1177/0883911505053658


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