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Nucleotide Isolation by Boronic Acid Functionalized Hydrogel Beads

Engineering Development Group, Roketsan Corporation, Elmadag, Ankara, Turkey, cicekh{at}superposta.com

Aminophenyl boronic acid (APBA) modified hydrogel beads were prepared as a new sorbent for nucleotide isolation. Spherical hydrogel beads, obtained by suspension copolymerization, were the base material for the sorbent. The carboxyl groups on the gel bead surface were activated with a water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). APBA was then covalently attached to the activated structure via the amine groups. The maximum APBA attached to the gel was 34mg/g. The reversible adsorption-desorption behavior of ß-nicotinamide adenine dinucleotide (ß-NAD) was investigated by using 3.5 and 34mg/g of APBA on the hydrogel beads. The equilibrium -NAD adsorption capacities for these beads were determined as approximately 25 and 100mg/g, respectively. The -NAD absorption capacity of these APBA beads is significantly greater than similar supports.

Key Words: boronate affinity chromatography • aminophenyl boronic acid • oligonucleotide • ß-nicotinamide adenine dinucleotide • hydroxypropyl methacrylate • suspension polymerization

Journal of Bioactive and Compatible Polymers, Vol. 20, No. 3, 245-257 (2005)
DOI: 10.1177/0883911505053376


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