Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents
Journal of Bioactive and Compatible Polymers
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Gürpinar, O. A.
Right arrow Articles by Onur, M. A.
Right arrow Search for Related Content
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

In Vitro Investigation of Cell Compatibility of Pure ß-TCP Granules

Ö. Aylin Gürpinar

M. Ali Onur

Hacettepe University, Faculty of Science, Department of Biology, Beytepe, 06532, Ankara, Turkey gurpinar{at}hacettepe.edu.tr

In this study, osteoblastic cells were isolated from rat bone marrow and characterized. The cells were cultured on ß-TCP granules and the osteoblast/ ß-TCP constructs. For this purpose, bone marrow was harvested under sterile conditions. Cell aggregates were broken up by pipetting and a cell suspension was cultured in DMEM/F12. After three days, the cells that adhered to the surface of the flask were cultured in osteoblast medium. When the cells became confluent, they were passaged and cultured in 24-well polystyrene cell culture dishes. Characterization of the osteoblasts, cell proliferation and alkaline phosphatase activity were measured on days 1, 7, 14, 21 and 30. To investigate the cell compatibility of the ß-TCP granules, osteoblastic cells were cultured on ß-TCP granules and a polystyrene cell culture dish (control group). Cell proliferation and alkaline phosphatase (ALP) activity were measured on days 1, 7, 14, 21 and 30 in both groups. Cell growth significantly increased at each time point, but on day 30 a decrease was observed. The ALP activity also increased at each time point and also decreased on day 30. This study may be regarded as the first step leading to a therapy for various bone defects.

Key Words: bone marrow • osteoblast • cell proliferation • ALP • osteocalcin • bone defects • tissue engineering

Journal of Bioactive and Compatible Polymers, Vol. 20, No. 2, 183-196 (2005)
DOI: 10.1177/0883911505051660
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?