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Collagen/Chitosan/Heparin Complex with Improved Biocompatibility for Hepatic Tissue Engineering

Department of Materials Science & Engineering, Tsinghua University, Beijing 100084, P.R. China

Alexandra Bichtelen

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China

Xiaohong Wang

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, wangxiaohong{at}tsinghua.edu.cn

Yongnian Yan

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China, dmeyyn{at}mail.Tsinghua.edu.cn

Feng Lin

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China

Zhuo Xiong

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China

Rendong Wu

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China

Renji Zhang

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China

Qingping Lu

Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, P.R. China

To make an implantable bioartificial liver (IBL), a new biocompatible collagen/chitosan/heparin complex was prepared using a crosslinking agent. The X-ray photoelectron spectroscopy (XPS), mechanical strength and biocompatibility with whole blood and hepatocytes were measured. The collagen/chitosan/heparin complex resulted in a superior blood compatibility compared to 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) crosslinked collagen matrix. The morphology and behavior of the cells on the collagen/chitosan/heparin membrane were found to be different from those on the collagen and collagen/chitosan membranes. Cells on the collagen membrane formed smaller three-dimensional aggregates than those on the collagen/chitosan membrane, while on the collagen/chitosan/heparin membrane, a round shape with no junctions were manifested. No adverse effects were found on the viability and function of the hepatocytes on the collagen/chitosan/heparin membrane compared to the collagen and collagen/chitosan membranes. These results suggest that this collagen/chitosan/heparin matrix is a potential candidate for hepatic tissue engineering.

Key Words: collagen/chitosan/heparin complex • biodegradable • biocompatibility • hepatocyte • scaffold • tissue engineering

Journal of Bioactive and Compatible Polymers, Vol. 20, No. 1, 15-28 (2005)
DOI: 10.1177/0883911505049653


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