Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Bioactive and Compatible Polymers
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Liao, S. S.
Right arrow Articles by Zhu, Y.
Right arrow Search for Related Content
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Osteoblasts Adherence and Migration through Three-dimensional Porous Mineralized Collagen Based Composite: nHAC/PLA

S. S. Liao

Biomaterials Laboratory Department of Material Science & Engineering Tsinghua University, Beijing 100084, China, Department of Dental Materials and Engineering School of Dentistry, Hokkaido University Sapporo 060-8586, Japan

F. Z. Cui

Biomaterials Laboratory Department of Material Science & Engineering Tsinghua University, Beijing 100084, China cuifz{at}mail.tsinghua.edu.cn

Y. Zhu

Biomaterials Laboratory Department of Material Science & Engineering Tsinghua University, Beijing 100084, China

Osteoblast cells were separated from the neonatal rat calvaria and co-cultured on a novel mineralized hydroxyapatite/collagen/poly(lactic acid) composite scaffold. By using this static cell culture, a three-dimensional osteoblasts/composite bone-like was constructed in vitro. The culture process was observed by scanning electron microscopy, fluorescence microscopy, confocal laser scanning microscopy, and histological analysis. Cells were observed to spread and proliferate throughout the inner-pores of the scaffold material. After a 12-day culture, the cells had grown into the interior scaffold about 200–400 µm depth of the composite by histological section observation. This mobile behavior of osteoblasts appeared to be similar to the composition and hierarchical structure of bone tissue. The adherence and migration of osteoblast cells in this three-dimensional composite is clinically important for large bone defect repair based on tissue engineering.

Key Words: tissue engineering • mineralized collagen • nano-hydroxyapatite • biomimetic • biodegradable • cell spreading • composite scaffold

Journal of Bioactive and Compatible Polymers, Vol. 19, No. 2, 117-130 (2004)
DOI: 10.1177/0883911504042643
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Journal of Bioactive and Compatible PolymersHome page
Y.J. Ren, X.D. Sun, F.Z. Cui, Y.T. Wei, Z.J. Cheng, and X.D. Kong
Preparation and Characterization of Antheraea pernyi Silk Fibroin Based Nanohydroxyapatite Composites
Journal of Bioactive and Compatible Polymers, September 1, 2007; 22(5): 465 - 474.
[Abstract] [PDF]