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Journal of Bioactive and Compatible Polymers
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In Vitro Cytocompatibility and Osteoinduction of Phosphorylated Chitosan with Osteoblasts

Y. Zhu

Xiaohong Wang

F. Z. Cui

cuifz{at}mail.tsinghua.edu.cn

Q. L. Feng

Biomaterials Laboratory Department of Materials Science & Engineering Tsinghua University, Beijing 100084, P.R. China

K. de Groot

Biomaterials Research Group, Leiden University Prof. Bronkhorstlaan 10, Building 57 3723 MB Bilthoven, The Netherlands

The cytocompatibility and osteoinduction of water-soluble phosphorylated chitosan (P-chitosan), that was modified by PO23 groups, with neonatal rat osteoblasts in vitro was studied. To identify the biocompatibility of P-chitosan with osteoblasts, the biofunctions of osteoblast cells, in the presence of three different concentrations of P-chitosan solutions as culture media and DMEM with 10% FBS as controls, were determined. The osteoblast differences in the P-chitosan solutions and on chitosan films were also investigated with immunocytochemistry. It was found, based on the ALP activity and mineralization assay that water-soluble P-chitosan has excellent cytocompatibility compared to the chitosan without phosphorylated modification. P-chitosan concentrations as high as 2% had a significant influence on cytocompatibility and osteoinduction; one tissue (or derived product) caused a second undifferentiated tissue to differentiate into bone. Water-soluble P-chitosan could be a promising osteoinductive biomaterial for tissue engineering and orthopedic uses.

Key Words: phosphorylated chitosan (P-chitosan) • cytocompatibility • osteoinduction • osteoblasts • bone forming

Journal of Bioactive and Compatible Polymers, Vol. 18, No. 5, 375-390 (2003)
DOI: 10.1177/0883911503039020
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