This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Wang, X. H. Articles by Ma, J. B. Search for Related Content Social Bookmarking                         What's this?

Responses of Osteo- and Fibroblast Cells to Phosphorylated Chitin

Biomaterial Laboratory, Department of Materials Science & Engineering Tsinghua University, Beijing 100084, P.R. China

Y. Zhu

Biomaterial Laboratory, Department of Materials Science & Engineering Tsinghua University, Beijing 100084, P.R. China

Q. L. Feng

Biomaterial Laboratory, Department of Materials Science & Engineering Tsinghua University, Beijing 100084, P.R. China

F. Z. Cui

Biomaterial Laboratory, Department of Materials Science & Engineering Tsinghua University, Beijing 100084, P.R. China

J. B. Ma

The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry Nankai University, Tianjin 300071, P.R. China

The cell responses of rat osteoblasts and fibroblasts to water-soluble phosphorylated chitin (P-chitin) were examined in vitro. Cell morphology was observed at 1, 4 and 7 days, respectively. A wide range of measurements, including the percentages of viable cells, total intracellular protein content, and 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrasodium bromide (MTT) assay on osteoblasts and fibroblasts, as well as alkaline phosphatase activity assay on osteoblasts at the end of 7 days culture, were conducted. P-chitin had no cytotoxity on either cell lines. An increase of viability and protein content of both cell types was found in media containing 0.10 g/mL of P-chitin after 7 days culture. The positive effects were especially significant for osteoblasts. Water-soluble P-chitin could be a promising biomaterial for tissue engineering.

Key Words: phosphorylated chitin (P-chitin) • cytocompatibility • fibroblasts • osteoblasts • cell culture.

Journal of Bioactive and Compatible Polymers, Vol. 18, No. 2, 135-146 (2003)
DOI: 10.1177/0883911503018002004


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?