This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ayhan, H. Search for Related Content Social Bookmarking                         What's this?

Model Protein BSA Adsorption and Covalent Coupling onto Methyl Methacrylate Based Latex Particles with Different Surface Properties

Hacettepe University, Chemical Engineering Department, Beytepe, Ankara, Turkeyhayhan{at}hacettepe.edu.tr

The adsorption and covalent coupling of bovine serum albumin (BSA) onto methylmethacrylate (MMA) based monodisperse latex particles with different hydrophylic surfaces were investigated. P(MMA) and P(MMA/HEMA) microbeads in the size range of 1.5–2.0 mm were prepared by a dispersion homopolymerization method in the presence of poly(vinylpyrrolidone) (PVP) and poly(vinyl alcohol) (PVA) as steric stabilizer and by copolymerization of MMA and 2-hydroxethyl methacrylate (HEMA) where PVP was used as steric stabilizers. Surface properties of these particles were characterized in terms of contact angle measurements and FTIR-DRS spectra. Additionally, hydroxyl groups of PMMA_PVA and P(MMA/HEMA) particles were activated to provide aldehyde groups on surfaces. Adsorption of the BSA onto these five types of latex particles was examined as a function of initial albumin concentration, pH and ionic strength of the various cations (Na+,Ca+ and Mg– and anions (Cl^– and SCN^–). The maximum adsorption capacities for all latex particles were obtained in solutions with 0.01µ m ionic strength and 1 mg/mL BSA concentration. When using PMMA_PVA, P(MMA/HEMA) and PMMA_PVP, the adsorption increased as hydrophobicity increased. Aldehyde activation of the two most hydrophobic particles elevated the coupling (immobilization) significantly in ionic strength of 0.01µ m. The adsorption and/or immobilization were maximum in the presence of monovalent cation, Na+ or anion, Cl–B for all types of latex particles.

Key Words: PMMA • P(MMA/HEMA) • BSA adsorption and coupling • different polymeric surfaces

Journal of Bioactive and Compatible Polymers, Vol. 17, No. 4, 271-283 (2002)
DOI: 10.1106/088391102026487


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?