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in Vivo Evaluation of Antibiotic Impregnated Beads in a Rat Osteomyelitis Model

Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Veterinary Teaching Hospital, 300 West Drake, Fort Collins, CO 80523.wdernell{at}vth.colostate.edu

C. Gentry-Weeks

Department of Pathology, College of Veterinary Medicine and Biomedical Science, Colorado State University, Fort Collins, CO, 80523

M. C. Manning

School of Pharmacy, University of Colorado Health Sciences Center, Denver, CO 80262

B. E. Powers

Department of Pathology, College of Veterinary Medicine and Biomedical Science, Colorado State University, Fort Collins, CO, 80523

R. D. Park

Radiological Health Sciences, College of Veterinary Medicine and Biomedical Science, Colorado State University, Fort Collins, CO 80523

M. Lafferty

C. A. Kuntz

Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University,Fort Collins, CO 80523

J. E. Shively

School of Pharmacy, University of Colorado Health Sciences Center, Denver, CO 80262

R. F. Falk

Department of Chemical Engineering, University of Colorado, Boulder, CO 80303

J. D. Meyer

School of Pharmacy, University of Colorado Health Sciences Center, Denver, CO 80262

T. W. Randolph

Department of Chemical Engineering, University of Colorado,

S. J. Withrow

Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University,Fort Collins, CO 80523

The biocompatibility, release and efficacy of gentamicin released from poly(lactic acid) (PLA) beads in an osteomyelitis model were evaluated. Microsphere particles of PLA containing gentamicin were compressed into 2.5 mm beads. Osteomyelitis was induced in the tibia by a gentamicin resistant Staphylococcus aureus in 32 Sprague-Dawley rats. Following debridement at 8 weeks, rats were randomized into four groups: 1) gentamicin-impregnated PLA bead implant, 2) PLA bead without gentamicin, 3) once-daily gentamicin injections (5 mg/kg) subcutaneously for 14 days, and 4) no treatment (control). Rats were sacrificed at 7 weeks, and the tibias were sampled for quantitative culture, histologic scoring and gentamicin concentration. Radiography and culture in 31 of the 32 rats confirmed osteomyelitis. A low level of gentamicin was measured in the serum of group 1 rats up to day 42. Gentamicin was not detectable in bone samples. There was no significant difference in radiographic scores, histologic scores or quantitative culture results between the groups. This study confirmed the consistency of this model, the biocompatibility of the PLA implants and the low level release of gentamicin from implanted beads.

Journal of Bioactive and Compatible Polymers, Vol. 16, No. 3, 235-250 (2001)
DOI: 10.1106/J42P-F4CF-H60Y-2P9K


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