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Study of Cell-Lipid Film Interaction by Measuring Heat Shock Proteins mRNA Expression

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065, Japan, kishida{at}ri.ncvc.go.jp

Takahisa Matsuyama

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065, Japan

Mihoko Nakashima

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065, Japan

Takeshi Serizawa

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065, Japan

Mitsuru Akashi

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065, Japan, akashi{at}apc.kagoshima-u.ac.jp

Kazuhisa Sugimura

Department of Bioengineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065, Japan

The adhesion and function of HeLa S3 cells on lipid films were investigated. The function of the cells adhering to lipid films was estimated by HSP 70B and HSP 47 mRNA expression using a reverse transcription-polymerase chain reaction (RT-PCR) analysis. It is apparent that HeLa S3 cells adhere to lipid films as well as tissue culture poly(styrene)(TCPS). HSPs mRNA expression on the lipid film depended on each lipid film. HeLa S3 cells adhered to the lipid films of DPPC, DPPE and DPPS expressed the same level HSPs mRNA as TCPS. High HSPs mRNA expressions were observed in the HeLa S3 cells adhering to films of synthetic lipids. The serum proteins affected the HSPs expression in the case of the synthetic lipids. These results imply that cell-material interactions are not simple and that mRNA expression is a powerful tool in the investigation of cell function, especially the reaction by weak stimulus such as cell-material contact.

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