This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Taguchi, T. Articles by Maruyama, I. Search for Related Content Social Bookmarking                         What's this?

Immobilization of Human Vascular Endothelial Growth Factor (VEGF165) onto Biomaterials: An Evaluation of the Biological Activity of Immobilized VEGF165

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan

Akio Kishida

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan

Mitsuru Akashi

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan

Ikuro Maruyama

Department of Clinical Laboratory Medicine, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan

Human vascular endothelial growth factors (VEGF) are angiogenic factors that induce specific endothelial cell proliferation. In this study, VEGF165, which contains 165 amino acids, was immobilized onto poly(acrylic acid) grafted polyethylene) films (PAAc-g-PE). VEGF165 was immobilized via a reaction between the amino group of VEGF165 and the carboxyl group of PAAc using water-soluble carbodiimide. Using cultured human umbilical vein endothelial cells (HUVEC), adhesion, proliferation, and migration of the cells were evaluated with three kinds of protein that were immobilized on the PE films. The proteins used were collagen (type IV), fibronectin (FN), and VEGF165. The adhesion of HUVEC was enhanced by the immobilization of collagen or FN and by the co-immobilization of VEGF with FN, but not VEGF alone. The VEGF FN-co-immobilized surface showed cell growth promotion activity. Endothelialization was observed only on the collagen-immobilized or VEGF FN-co-immobilized film surfaces. We proposed that the VEGF with FN-co-immobilized biomaterials could be used for artificial vessels and for other tissue engineering scaffolds.

Journal of Bioactive and Compatible Polymers, Vol. 15, No. 4, 309-320 (2000)
DOI: 10.1177/088391150001500403


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?