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Journal of Bioactive and Compatible Polymers
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Effect of Polyethylene Oxide Blocks or Grafts on the Physicochemical Properties of Poly(2-N-(Dimethylaminoethyl) Methacrylate) DNA Complexes

Peter Dubruel

Polymer Materials Research Group, Department of Organic Chemistry, University of Ghent, Ghent 9000, Belgium

Etienne H. Schacht

Polymer Materials Research Group, Department of Organic Chemistry, University of Ghent, Ghent 9000, Belgium, etienne.schacht{at}rug.ac.be

The synthesis and the physicochemical evaluation of a series of polyethylene oxide (PEO) containing polyamines are described as potential vectors in gene therapy. Two different types of vectors were synthesized: PEO block copolymers and copolymers containing PEO grafts. The building unit of the polyamine is 2-N-(dimethylaminoethyl) methacrylate. All polymers are able to condense DNA according to dynamic light scattering measurements and ethidium bromide exclusion tests. Complexes of PEO block copolymers and DNA show a stability comparable to that of the homopolymer poly(2-N-(dimethylaminoethyl) methacrylate) (PDMAEMA). Polymers with a PEO graft show a lower stability compared to the unmodified polymer. Incorporation of a PEO graft resulted in a decrease in the zeta potential of the complexes at all charge ratios. The interaction of a serum albumin with complexes of DNA and PEO containing polymers was studied at different protein concentrations. Polymers with a PEO graft show a lower interaction with serum albumin compared to the homopolymer based on turbidity measurements.

Journal of Bioactive and Compatible Polymers, Vol. 15, No. 4, 279-296 (2000)
DOI: 10.1177/088391150001500401
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