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In Vitro Calcification Model (2): Apatite Formation on Segmented Polyurethane Thin Films by Using an Alternate Soaking Process: The Effect of Adsorbed Serum Proteins on Calcification

Department of Biomedical Engineering, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Kou Imachi

Department of Biomedical Engineering, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Tetsushi Taguchi

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan

Mitsuru Akashi

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan

Kimiyasu Sato

National Institute for Research in Inorganic Materials, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

Junzo Tanaka

National Institute for Research in Inorganic Materials, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

In the present study, the effect of serum proteins on the calcification (apatite deposition) of commercially available polyurethane (PU) films, Pellethane® 2363-80AE, Pellathane® 2363-55DE, and K-III, was evaluated by using an alternating soaking process. Bovine serum albumin, -globulin and fibrinogen were used as typical serum proteins. After the proteins were adhered to PU films, the films were used in the apatite formation process. The thin-film X-ray diffraction spectra of a fibrinogen adhered K-III sample, after 200 calcification sequences, showed HAp formation. The calcium content on the surface of all films to which protein adhered was greater than that of films incubated in protein free solutions. The films to which fibrinogen adhered showed remarkable apatite formation, though the amount of adhered fibrinogen was much smaller than the amounts of the other proteins. K-III films to which fibrinogen had adhered showed small globular apatite aggregations, whereas the Pellethane® 2363 series films showed confetti-shaped apatite. Different morphologies exhibited by the deposits depended on the types of serum protein and on the substrate chemistry. The reason for the different apatite morphology on different PU films is not clear, however, the kind of serum protein seems to play an important role in the calcification of a PU.

Journal of Bioactive and Compatible Polymers, Vol. 15, No. 3, 230-244 (2000)
DOI: 10.1177/088391150001500303


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