This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ayhan, H. Articles by Piskin, E. Search for Related Content Social Bookmarking                         What's this?

Protein A Immobilized Poly(Methylmethacrylate-Co-Hydroxyethylmethacrylate) Microbeads for IgG Adsorption

Hacettepe University, Chemical Engineering Department, Bioengineering Division and TUBITAK-Center of Excellence: Polymeric Biomaterials, Beytepe, P.K. 716 Kizilay, Ankara, Turkey

V. Bulmus

Hacettepe University, Chemical Engineering Department, Bioengineering Division and TUBITAK-Center of Excellence: Polymeric Biomaterials, Beytepe, P.K. 716 Kizilay, Ankara, Turkey

E. Piskin

Hacettepe University, Chemical Engineering Department, Bioengineering Division and TUBITAK-Center of Excellence: Polymeric Biomaterials, Beytepe, P.K. 716 Kizilay, Ankara, Turkey

Poly(methylmethacrylate-co-2-hydroxyethylmethacrylate) microbeads in the size range of 1.5-2.0µm were prepared by a phase inversion polymerization. The hydroxyl groups were activated by periodate oxidation, and the active ligand, i.e., protein A was immobilized via a spacer-arm, i.e., hexamethylene diamine (HDMA) by using a cross-linker, i.e., glutaraldehyde and protein A. The optimal concentration obtained for modifications are as follows: sodium periodate concentration: 0.467 x 10^–2mmol/mL; HMDA concentration: 3.5 x 10^–2mmol/mL; and glutaraldehyde concentration: 0.7 x 10^–6mmol/mL. Yields of immobilization of protein A onto the plain and periodate oxidized microbeads were found very close, and were in the range of 0.01-0.02 mg protein A/g microbeads. The optimal conditions for immobilization are as follows: the initial protein A concentration: 0.1 mg/mL; temperature: 25°C; pH: 9.5; and immobilization time:120 min. Incorporation of protein A at these conditions resulted in 0.825 mg protein A/g microbeads. The HIgG adsorption onto these protein A incorporated microbeads was 41 mg HIgG/g microbeads.

Journal of Bioactive and Compatible Polymers, Vol. 14, No. 6, 490-503 (1999)
DOI: 10.1177/088391159901400604


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?