This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Sakamoto, N. Articles by Onishi, M. Search for Related Content Social Bookmarking                         What's this?

Synthesis and Anticoagulant Properties of a Novel Heparinoid N-Sulfate-Bearing Vinylpolymer

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan

T. Yumura

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan

M. Akashi

Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan

M. Onishi

R&D Department, Terumo Corporation, 1500 Inokuchi, Nakai-machi, Ashigarakami-gun, Kanagawa 259-0151, Japan

A novel heparinoid polymer was prepared with an N-sulfate side chain group by sulfation of poly(N-vinylacetamide-co-vinylamine) [poly(NVA-co-VAm)] which was obtained by the partial hydrolysis of poly(N-vinylacetamide) [poly(NVA)]. The hydrolysis of poly(NVA) was controlled by reaction time and temperature and the sulfation of poly(NVA-co-VAm) was performed with chlorosulfonic acid. Poly(N-vinylacetamide-co-vinylamine-co-vinylsulfamic acid) [poly(NVA-co-VAm-co-VSA)] was confirmed by means of IR spectroscopy, and the degree of sulfation of poly(NVA-co-VAm-co-VSA) was estimated by elemental analysis. The anticoagulant properties of poly(NVA-co-VAm-co-VSA) were evaluated using the Lee-White clotting test and activated partial thromboplastin time (APTT) test. The human whole blood clotting time in the presence of poly(NVA-co-VAm-co-VSA) was prolonged compared with poly(NVA) and poly(NVA-co-VAm). The clotting time was prolonged with increased N-sulfate groups on the polymer. APTT tests of the polymer were also prolonged with N-sulfate increases. The results suggest that the N-sulfate components on the polymer can endow anticoagulant activity to synthetic polymers.

Journal of Bioactive and Compatible Polymers, Vol. 14, No. 2, 150-161 (1999)
DOI: 10.1177/088391159901400204


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?