This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Pandit, A. Articles by Thompson, A. Search for Related Content Social Bookmarking                         What's this?

The Effect on Wound Healing by a Modified Fibrin Scaffold Delivering Acidic Fibroblast Growth Factor (FGF-1)

University of Alabama at Birmingham, Birmingham, AL 35294

Dale Feldman

University of Alabama at Birmingham, Birmingham, AL 35294

Catherine Listinsky

University of Alabama at Birmingham, Birmingham, AL 35294

Anthony Thompson

University of Alabama at Birmingham, Birmingham, AL 35294

This project investigated the efficacy of modified fibrin scaffold as a vehicle for acidic fibroblast growth factor (FGF-1). The goal of this study was to justify the use of a modified fibrin scaffold with altered surface roughness and porosity as a drug-delivery matrix, specifically for the delivery of FGF-1. The hypothesis tested was that FGF-1 delivered in an optimal dose through a modified fibrin scaffold would result in a significant increase in angiogenesis, epithelialization, fibroblast, and healing rate, and correspond to a decrease in overall healing time. In vivo testing was conducted in a full-thickness defect model in a rabbit. Four 3 x 3-cm full-thickness defects were created on the dorsum of each of five white New Zealand rabbits. There were four treatments to be investigated: modified fibrin scaffold, modified fibrin/FGF-1 scaffolds, topical FGF-1, and control defects. The regimen of topical dosing of FGF-1 was determined from release kinetic studies using the modified fibrin scaffold. The areas and perimeters of the initial wound and final wounds were obtained from histological slides using an image analysis system. Standard histomorphometric techniques were used to characterize the wound-healing response. In this study, the modified fibrin/FGF-1 scaffold served as a suitable vehicle for FGF-1, providing an enhanced angiogenic and a fibroblastic healing response and, thus, was found to be an effective scaffold.

Journal of Bioactive and Compatible Polymers, Vol. 12, No. 2, 99-111 (1997)
DOI: 10.1177/088391159701200202


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?