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Sensitization of Nude Mice Using Direct Liposome Transfer of Tumor Cell Antigens

Osamu Ike

Shigeki Hitomi

Hiromi Wada

Department of Thoracic Surgery, Chest Disease Research Institute, Kyoto University, 53, Shogoin Kawaharacho, Sakyo-ku, Kyoto, Japan

Yukihisa Okumura

Junzo Sunamoto

Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Kyoto, Japan

Transplantable human squamous cell carcinoma cells were coincubated with 1,2-dimyristoylamido-1,2-deoxyphosphatidyl-choline (D14DPC) liposomes. These proteoliposomes and disrupted tumor cell suspensions were injected subcutaneously into two groups of nude mice, respectively, while the third group was untreated. Ten days previously, tumor had been implanted into both of the mice forefeet. In the seven-week old mice pretreated with the proteoliposomes, one of the tumors disappeared, while another tumor remained unchanged in size and the remaining two tumors regrew. Regrowth of the tumors was observed in both control groups. Their size and weight were significantly larger (p < 0.05) than those in the proteoliposome group. In all the 5-week old mice, the tumor masses grew but the size and weight of the tumor in the proteoliposome treated group were significantly smaller (p < 0.05) than those in the other two groups. The results showed the effectiveness of the proteoliposome treatment as a possible clinical application.

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