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The Application of Luminol-Bound Microspheres for the Quantitative Analysis of Toxic Oxygen within Phagosomes

Basic Research Laboratories Toray Industries, Inc. 1111 Tebiro, Kamakura 248, Japan

Sanae Masuko

Basic Research Laboratories Toray Industries, Inc. 1111 Tebiro, Kamakura 248, Japan

Shuntaro Hosaka

Basic Research Laboratories Toray Industries, Inc. 1111 Tebiro, Kamakura 248, Japan

Hiroshi Tanzawa

Basic Research Laboratories Toray Industries, Inc. 1111 Tebiro, Kamakura 248, Japan

Hydrophilic microspheres, with a diameter of about 2µm, composed of glyceryl methacrylate and methacrylic acid were prepared. The microspheres could be stably stored in dispersed state and demonstrated little non-specific adherance to cell membranes. Luminol-bound microspheres, prepared by covalently binding luminol to microspheres, were phagocytosed by phagocytic cells without opsonization. Luminol bound to the microspheres was oxidized by toxic oxygen within phagosomes resulting in the generation of chem iluminescence. The chemiluminescence elicited by luminol-bound micro spheres was an effective method of measuring the candidacidal activity of the cells. The difference in phagosomal toxic oxygen versus extracellular toxic oxy gen was examined.

Journal of Bioactive and Compatible Polymers, Vol. 1, No. 2, 172-180 (1986)
DOI: 10.1177/088391158600100204


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